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antigen retrieval immunohistochemistry, formalin fixed paraffin embedded, immunohistochemistry protocol, antigen recovery, antigen retrieval buffer, tissue sections, antigen unmasking, antigen retrieval protocol, antigen retrieval pressure cooker, antigen retrieval microwave, antigen retrieval methods, epitope retrieval, antibody, tissue section, immunohistochemistry, paraffin, formalin, citrate buffer, pathology, ihc
A reliable, time tested solution to unmask antigen on formalin-fixed sections
Aptum Biologics Ltd.
38 Baker's Drove
Southampton
SO16 8AD
United Kingdom
carbonate buffer elisa
elisa coating buffer ph
elisa plate coating buffer
elisa coating buffer sodium bicarbonate
pbs as coating buffer for elisa
elisa coating buffer pbs
elisa carbonate coating buffer
coating buffer
carbonate coating buffer
antibody coating buffer
elisa plate coating
coating elisa plates at 37 degrees
antibody coating on elisa plate
elisa coating plate
coating in elisa
coating antibody for elisa
elisa antibody coating
elisa peptide coating
Coating Stabilisers
This is a group of reagents for stabilizing the coated antigens or antibodies on assay plates. They can can be successfully used for stabilizing antigens and antibodies on glass on polystyrene surfaces for protein arrays. Plates (or arrays, or chromatographic strips - lateral flow assays) treated with Plate Sealer can be stored for long periods of time without changes in the properties of applied antigen of antibody.
These reagents are developed and made for:
- producers of commercial assays that have to be stored for a prolonged time
- plates coated for experiments to be performed over a long period of time or to be used later "in the filed".
Description & Properties
If you want to store plates with a coated antigen or antibody for a prolonged period of time, you may do so by freezing them at -20 C, but often the freezing-thawing step is highly undesirable, especially when coated molecules are antibodies or antigens with a complex structure. Stabilizing the molecules coated on the pate becomes even more necessary when you plan to store them for a prolonged period of time (e.g. diagnostic kits that have to be shipped overseas), stabilization can save the coated molecules when the storage temperature cannot be guaranteed.
Plate Sealer seals the plate with a uniform layer, which is well soluble and does not affect the results of the assay. Plates treated with Plate Sealer and dried can be dried and stored for long periods. The recommended optimal storage temperature is +2-8°C, but the plates in most cases can be stored dry at ambient temperatures for many months without the applied antigens/antibody losing any activity.
Ordering codes
We offer two versions of Plate Sealer, a regular sealer and its AF (animal-free) version that does not contain any components of animal origin.
Plate Sealer Ready-to-use 50 ml #AP0522-050
125 ml #AP0522-125
500 ml #AP0522-500
Plate Sealer-AF Ready-to-use 50 ml #AP0523-050
125 ml #AP0523-125
500 ml #AP0523-500
Using Coating Stabilisers (general guide)
- add 200 μl/well Plate Sealer
- incubate 15-90 minutes at room temperature
- remove solution (do not rinze!)
- dry the plates 1-2 hours at 37-45°C
Plates can be stored dry, sealed in a pouch, for 1-3 years (2-8°C).