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Antigen retriever, immunohistochemistry, tissue sections, antigen recovery, formalin-fixed, paraffin embedded tissue sections, immunohistochemistry protocols

​​Antigen retriever, immunohistochemistry, tissue sections, antigen recovery, formalin-fixed, paraffin embedded tissue sections, immunohistochemistry protocols

​​​Antigen retriever, immunohistochemistry, tissue sections, antigen recovery, formalin-fixed, paraffin embedded tissue sections, immunohistochemistry protocols

​​​Antigen retriever, immunohistochemistry, tissue sections, antigen recovery, formalin-fixed, paraffin embedded tissue sections, immunohistochemistry protocols

​​​Antigen retriever, immunohistochemistry, tissue sections, antigen recovery, formalin-fixed, paraffin embedded tissue sections, immunohistochemistry protocols

​​​Antigen retriever, immunohistochemistry, tissue sections, antigen recovery, formalin-fixed, paraffin embedded tissue sections, immunohistochemistry protocols

​​​Antigen retriever, immunohistochemistry, tissue sections, antigen recovery, formalin-fixed, paraffin embedded tissue sections, immunohistochemistry protocols

​​​Antigen retriever, immunohistochemistry, tissue sections, antigen recovery, formalin-fixed, paraffin embedded tissue sections, immunohistochemistry protocols

​​​Antigen retriever, immunohistochemistry, tissue sections, antigen recovery, formalin-fixed, paraffin embedded tissue sections, immunohistochemistry protocols

​​​Antigen retriever, immunohistochemistry, tissue sections, antigen recovery, formalin-fixed, paraffin embedded tissue sections, immunohistochemistry protocols

​​​Antigen retriever, immunohistochemistry, tissue sections, antigen recovery, formalin-fixed, paraffin embedded tissue sections, immunohistochemistry protocols

​​​Antigen retriever, immunohistochemistry, tissue sections, antigen recovery, formalin-fixed, paraffin embedded tissue sections, immunohistochemistry protocols

​​​Antigen retriever, immunohistochemistry, tissue sections, antigen recovery, formalin-fixed, paraffin embedded tissue sections, immunohistochemistry protocols

​​​Antigen retriever, immunohistochemistry, tissue sections, antigen recovery, formalin-fixed, paraffin embedded tissue sections, immunohistochemistry protocols

​​​Antigen retriever, immunohistochemistry, tissue sections, antigen recovery, formalin-fixed, paraffin embedded tissue sections, immunohistochemistry protocols

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Advantages of Retriever
Retriever preserves tissue morphology

Thanks to its precisely calibrated pressure and heating cycle, the Retriever effectively avoids buffer boiling in the chambers, thus preventing the formation of air bubbles that can negatively impact tissue morphology. In the images below, we present a comparison of unprocessed intestinal tissue, tissue processed in a microwave (representing the standard result), and tissue processed using the Retriever. It is worth noting that even the mucus produced by intestinal cells remains fully unaffected, and the cells maintain their proper morphology. The cell walls and nucleus exhibit the same shape as seen in the unprocessed section.

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cell morphology after Retriever

This is a section of intestinal tissue fixed with formalin and embedded into paraffin

Processing the tissue in microwave-type machines results in damage of the morphology of the gentle tissues.

No damage to morphology occurs, however, after processing the tissue in Retriever, although the unmasking is usually better than after the microwave

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Excellent Staining and Excellent Tissue Morphology

The unaffected morphology of tissues processed in the Retriever does not imply poor processing. As demonstrated in the images below, both cell surface antigens and nuclear antigens are effectively detected by antibodies. On the right side, you can observe a test staining of cervix tissue using an anti-E-cadherin antibody. Additionally, stainings for CD8, Ki67, and PCNA are displayed below. Different buffers, such as citrate, acid citrate, and EDTA, were used for these processes. Please take note of the preserved tissue morphology, as well as the intensity of staining without any noticeable background interference.

E-Cadherin (mAb) → 

Stratified epithelium in the Cervix expressing E-cadherin, an intercellular adhesion molecule. Please notea strong and crisp staining at intercellular junctions.

E-cadgerin staining of FF/PE sectioms after processing in 2100 Retriever

​CD8 (mAb)

Infiltrating cytotoxic CD8+  T- cells were detected in formalin fixed, paraffin embedded section of human intestine after antigen unmasking in Retriever. Buffer B was used for processing.

​PCNA (mAb)

Marker for proliferating cells is shown for cells of human intestine. Note the morphology of the tissue that is completely unchanged by processing.

​Ki-67 (antibody MIB1)

Routine proliferation marker detected on formalin-fixed paraffin embedded section of human intestine. Please note the strong nuclear staining, extremely clean background (no non-specific binding of the antibody) and excellent morphology of the tissue including the preservation of the mucosal secret in the cells and undamaged membranes. Buffer A was used for processing.

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